2YKD
Structure of the matrix protein from human respiratory syncytial virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.402, 78.902, 65.827 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 25.190 - 1.860 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vqp |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.658 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.200 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 22370 | |
| <I/σ(I)> | 7.1 | 1.3 |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291 | MOTHER LIQUOR 45 % TO 65% TACSIMATE PH7, 18 DEGC, PROTEIN AT 1 MG/ML |
