2YK6
Structure of Neisseria LOS-specific sialyltransferase (NST), in complex with CDP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.099, 123.510, 41.652 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.710 - 2.830 |
| R-factor | 0.19294 |
| Rwork | 0.190 |
| R-free | 0.25654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yk5 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.694 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.710 | 2.880 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.121 | 0.522 |
| Number of reflections | 11162 | |
| <I/σ(I)> | 15.4 | 2.7 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 6.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.4 | PROTEIN WAS CRYSTALLIZED BY VAPOR DIFFUSION METHODS USING DROPS OF PROTEIN MIXED WITH AN EQUAL VOLUME OF PRECIPITANT: 100 MM SODIUM ACETATE (PH 4.2 - 4.4) 1.7 M DI-AMMONIUM SULFATE. |
