2YK5

Structure of Neisseria LOS-specific sialyltransferase (NST), in complex with CMP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 2
Unit cell lengths	85.747, 124.137, 41.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.530 - 2.320
R-factor	0.17881
Rwork	0.176
R-free	0.22334
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.020
RMSD bond angle	1.681
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.530	2.410
High resolution limit [Å]	2.330	2.330
Rmerge	0.010	0.040
Number of reflections	19839
<I/σ(I)>	17	3.89
Completeness [%]	100.0	100
Redundancy	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.4		PROTEIN WAS CRYSTALLIZED BY VAPOR DIFFUSION METHODS USING DROPS OF PROTEIN MIXED WITH AN EQUAL VOLUME OF PRECIPITANT: 100 MM SODIUM ACETATE (PH 4.2 - 4.4) 1.7 M DI-AMMONIUM SULFATE