2YK0
Structure of the N-terminal NTS-DBL1-alpha and CIDR-gamma double domain of the PfEMP1 protein from Plasmodium falciparum varO strain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-22 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.907, 144.362, 75.836 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 40.230 - 2.800 |
| R-factor | 0.2148 |
| Rwork | 0.213 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.250 | 1.000 |
| Number of reflections | 39458 | |
| <I/σ(I)> | 6.9 | 0.8 |
| Completeness [%] | 96.9 | 84.7 |
| Redundancy | 5.4 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | VAPOUR DIFFUSION, 10 MG/ML PROTEIN AGAINST 10% PEG 3350, 200 MM NACL, 100 MM SODIUM CITRATE PH 8.3 |
