2YJV

Crystal structure of E. coli regulator of ribonuclease activity A (RraA) bound to fragment of DEAD-box protein RhlB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-07
Detector	ADSC CCD
Spacegroup name	C 1 2 1
Unit cell lengths	259.630, 69.070, 123.300
Unit cell angles	90.00, 109.17, 90.00

Refinement procedure

Resolution	122.620 - 2.800
R-factor	0.22434
Rwork	0.221
R-free	0.28774
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q5x
RMSD bond length	0.014
RMSD bond angle	1.507
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.530	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.050	0.260
Number of reflections	47500
<I/σ(I)>	15.5	3.7
Completeness [%]	98.0	99.3
Redundancy	2.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	298	CRYSTALS WERE OBTAINED USING THE HANGING DROP METHOD, BY MIXING IN 1 TO 1 RATIO PROTEIN SAMPLE WITH MOTHER LIQUOR 100 MM SODIUM CITRATE PH 5.4, 32% MPD AND 200 MM AMMONIUM ACETATE AT 25 C. PLATES WERE THEN IMMEDIATELY TRANSFERRED TO 16 C. CRYSTALS WERE DIRECTLY FLASH FROZEN IN LIQUID NITROGEN.