2YJV
Crystal structure of E. coli regulator of ribonuclease activity A (RraA) bound to fragment of DEAD-box protein RhlB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-07 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 259.630, 69.070, 123.300 |
Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
Resolution | 122.620 - 2.800 |
R-factor | 0.22434 |
Rwork | 0.221 |
R-free | 0.28774 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q5x |
RMSD bond length | 0.014 |
RMSD bond angle | 1.507 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.530 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.050 | 0.260 |
Number of reflections | 47500 | |
<I/σ(I)> | 15.5 | 3.7 |
Completeness [%] | 98.0 | 99.3 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | CRYSTALS WERE OBTAINED USING THE HANGING DROP METHOD, BY MIXING IN 1 TO 1 RATIO PROTEIN SAMPLE WITH MOTHER LIQUOR 100 MM SODIUM CITRATE PH 5.4, 32% MPD AND 200 MM AMMONIUM ACETATE AT 25 C. PLATES WERE THEN IMMEDIATELY TRANSFERRED TO 16 C. CRYSTALS WERE DIRECTLY FLASH FROZEN IN LIQUID NITROGEN. |