2YJM
Structure of TtrD from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-28 |
| Detector | RIGAKU CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.160, 37.930, 49.120 |
| Unit cell angles | 90.00, 94.16, 90.00 |
Refinement procedure
| Resolution | 48.990 - 1.840 |
| R-factor | 0.21575 |
| Rwork | 0.214 |
| R-free | 0.24515 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2idg |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.984 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.010 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.160 |
| Number of reflections | 13256 | |
| <I/σ(I)> | 9.9 | 4.3 |
| Completeness [%] | 90.6 | 84.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN BUFFER: 50 MM TRIS, 250 MM NACL, 5 MM GMP-PNP; RESERVOIR: 0.1 M CHES PH 9.5, 20% W/V PEG 8K. |
