2YJJ
Structure of Dps from MICROBACTERIUM ARBORESCENS in the low iron form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.530, 91.920, 128.450 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 29.010 - 2.050 |
| R-factor | 0.17382 |
| Rwork | 0.171 |
| R-free | 0.22574 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9r |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.774 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.100 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.080 | 0.790 |
| Number of reflections | 120300 | |
| <I/σ(I)> | 7.3 | 1.8 |
| Completeness [%] | 99.4 | 97.9 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 18% PEG8000, 2% ISO-PROPANOL, 0.1 M NA-ACETATE, PH 7.5 |
