2YJE

Oligomeric assembly of actin bound to MRTF-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-10
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	90.935, 90.935, 321.613
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.883 - 3.100
R-factor	0.2371
Rwork	0.235
R-free	0.27980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v52
RMSD bond length	0.012
RMSD bond angle	1.115
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.050	0.330
Number of reflections	24795
<I/σ(I)>	12.4	2.3
Completeness [%]	97.5	97.5
Redundancy	6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.25		0.1 M BTP PH 8.25, 20.5% PEG 3350, 0.2 M SODIUM NITRATE.