2YIZ
X-ray structure of Mycobacterium tuberculosis Dodecin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-17 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 94.302, 94.302, 230.907 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.910 - 1.700 |
| R-factor | 0.17261 |
| Rwork | 0.172 |
| R-free | 0.19743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v21 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.342 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.910 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.780 |
| Number of reflections | 43832 | |
| <I/σ(I)> | 24.8 | 3.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 11.1 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 10 MM CO CL2, 100 MM NAOAC PH 5.0, 200 MM HEXANEDIOL. |
