2YIH
Structure of a Paenibacillus polymyxa Xyloglucanase from GH family 44 with Xyloglucan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2010-09-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 83.530, 83.530, 157.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.760 - 1.700 |
| R-factor | 0.15818 |
| Rwork | 0.157 |
| R-free | 0.18418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ykk |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.760 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 67177 | |
| <I/σ(I)> | 12 | 3.1 |
| Completeness [%] | 95.8 | 93.2 |
| Redundancy | 5.3 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 27% (W/V) PEG 3350, 0.2 M LITHIUM SULPHATE, 0.1 M BIS TRIS PH 6.5. |
