2YIB

Structure of the RNA polymerase VP1 from Infectious Pancreatic Necrosis Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-16
Detector	ADSC QUANTUM 4r
Spacegroup name	P 21 21 21
Unit cell lengths	119.740, 195.450, 197.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.430 - 3.800
R-factor	0.1858
Rwork	0.184
R-free	0.21290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yi9
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	4.010
High resolution limit [Å]	3.800	3.800
Rmerge	0.300	0.820
Number of reflections	45508
<I/σ(I)>	4.5	1.8
Completeness [%]	98.5	99
Redundancy	4.9	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP			CRYSTALS OF FULL-LENGTH VP1 WERE GROWN IN SITTING DROPS CONTAINING 100 NL PROTEIN (5.3 MG/ML) PLUS 5 MM MNCL2 AND 100 NL RESERVOIR SOLUTION (20% W/V PEG3350, 0.1 M BIS-TRIS PROPANE PH 7.5, 0.2 M SODIUM CITRATE, 20 MM ATP, 5% V/V MPD, 10 MM NAOH) EQUILIBRATED AGAINST 95 UL RESERVOIRS AT 20.5 C.