2YHT

Crystal structure of Hfq riboregulator from E. coli (P1 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Spacegroup name	P 1
Unit cell lengths	61.200, 61.200, 53.100
Unit cell angles	82.60, 87.30, 60.00

Refinement procedure

Resolution	30.566 - 2.900
R-factor	0.1833
Rwork	0.181
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y90
RMSD bond length	0.009
RMSD bond angle	1.154
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.140
Number of reflections	14455
<I/σ(I)>	12	6.5
Completeness [%]	97.4	82.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.4	293	CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL SITTING DROPS. THE RESERVOIR CONTAINED 30% PEG 3350, 0.1 M NA-CITRATE PH 5.4. CRYSTALLIZATIONS WERE CARRIED OUT AT 20 DEGREES CELSIUS.