2YHS

Structure of the E. coli SRP receptor FtsY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-27
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 2
Unit cell lengths	79.358, 108.412, 31.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.679 - 1.600
R-factor	0.1803
Rwork	0.178
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fts
RMSD bond length	0.006
RMSD bond angle	1.006
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.700	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.100	0.590
Number of reflections	38500
<I/σ(I)>	12.8	2.7
Completeness [%]	99.4	97.1
Redundancy	6.1	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.1 M HEPES PH 7.0, 30% JEFFAMINE ED-2001 PH 7.0.