2YHN
The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | ADSC CCD |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 79.260, 22.770, 87.838 |
| Unit cell angles | 90.00, 116.82, 90.00 |
Refinement procedure
| Resolution | 35.370 - 3.000 |
| R-factor | 0.232 |
| Rwork | 0.228 |
| R-free | 0.29700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eb5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.842 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.190 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | 0.340 |
| Number of reflections | 2928 | |
| <I/σ(I)> | 12.2 | 7.4 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M SODIUM ACETATE PH 7-8, 16-20% MPD. |
