2YHF

1.9 Angstrom Crystal Structure of CLEC5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX10.1
Synchrotron site	SRS
Beamline	PX10.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-02
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 31
Unit cell lengths	109.109, 109.109, 84.879
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	94.490 - 1.900
R-factor	0.219
Rwork	0.216
R-free	0.26727
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1K9J 3bdw 1e87 2it5 3BC6.
RMSD bond length	0.007
RMSD bond angle	0.947
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.3.0002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.500
Number of reflections	88632
<I/σ(I)>	12.9	1.62
Completeness [%]	87.1	76.4
Redundancy	2.9	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		100MM TRIS PH8.0, 30% PEG 6000