2YHB
Crystal Structure of the N. crassa QDE-2 AGO MID-PIWI Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 218.910, 218.910, 76.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.730 - 3.650 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2XDY AND 3DLB |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.730 | 3.750 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Number of reflections | 12017 | |
| <I/σ(I)> | 9.14 | 2.18 |
| Completeness [%] | 96.1 | 97.1 |
| Redundancy | 3.23 | 3.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 200 MM POTASSIUM FORMATE, 1.7 M AMMONIUM SULFATE., pH 7.2 |
