2YHA

Crystal Structure of the N. crassa QDE-2 AGO MID-PIWI Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-19
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 32 2 1
Unit cell lengths	63.080, 63.080, 170.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.629 - 1.850
R-factor	0.1972
Rwork	0.196
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yhb
RMSD bond length	0.015
RMSD bond angle	1.453
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.600	1.940
High resolution limit [Å]	1.850	1.850
Number of reflections	34431
<I/σ(I)>	15.3	2.39
Completeness [%]	99.8	100
Redundancy	5.07	5.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.2		200 MM DI-SODIUM TARTRATE, 2.3 M AMMONIUM SULFATE., pH 7.2