2YH9
Crystal structure of the dimeric BamE from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.660, 96.520, 50.490 |
| Unit cell angles | 90.00, 134.06, 90.00 |
Refinement procedure
| Resolution | 22.290 - 1.800 |
| R-factor | 0.18366 |
| Rwork | 0.181 |
| R-free | 0.22859 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.831 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.470 |
| Number of reflections | 21093 | |
| <I/σ(I)> | 25.7 | 2.8 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG 4000, 10% I-PROH, 0.1 M HEPES PH 7.5 |
