2YH6
Structure of the N-terminal domain of BamC from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.577, 46.631, 60.208 |
| Unit cell angles | 102.70, 92.86, 118.20 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.19676 |
| Rwork | 0.195 |
| R-free | 0.23430 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.530 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 56570 | |
| <I/σ(I)> | 11.1 | 2.7 |
| Completeness [%] | 95.1 | 93.8 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 | 2 M (NH4)2SO4, 0.1 M CITRIC ACID PH 3.5 |
