2YGU
Crystal structure of fire ant venom allergen, Sol I 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.211, 139.160, 62.093 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.23887 |
| Rwork | 0.237 |
| R-free | 0.27046 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.005 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.660 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 28662 | |
| <I/σ(I)> | 17.3 | 3.8 |
| Completeness [%] | 95.0 | 86.3 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.2 | PROTEIN WAS CRYSTALLIZED FROM 35% PEG 8000, 0.1M NAACETATE, PH 9.2 . |
