2YGU
Crystal structure of fire ant venom allergen, Sol I 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-09 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.211, 139.160, 62.093 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.600 |
R-factor | 0.23887 |
Rwork | 0.237 |
R-free | 0.27046 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.005 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.660 | 2.720 |
High resolution limit [Å] | 2.580 | 2.580 |
Rmerge | 0.060 | 0.280 |
Number of reflections | 28662 | |
<I/σ(I)> | 17.3 | 3.8 |
Completeness [%] | 95.0 | 86.3 |
Redundancy | 3.2 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9.2 | PROTEIN WAS CRYSTALLIZED FROM 35% PEG 8000, 0.1M NAACETATE, PH 9.2 . |