2YGQ
WIF domain-epidermal growth factor (EGF)-like domains 1-3 of human Wnt inhibitory factor 1 in complex with 1,2- dipalmitoylphosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 178.007, 178.007, 178.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.804 - 3.951 |
| R-factor | 0.2985 |
| Rwork | 0.297 |
| R-free | 0.33060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.087 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 4.090 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.100 | 0.870 |
| Number of reflections | 8403 | |
| <I/σ(I)> | 27.2 | 2.8 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 11.1 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 0.75 M SODIUM SUCCINATE PH 5.7, 35 MG/ML SUCROSE OCTASULFATE |
