2YGN

WIF domain of human Wnt inhibitory factor 1 in complex with 1,2- dipalmitoylphosphatidylcholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	C 2 2 2
Unit cell lengths	49.912, 134.138, 60.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.529 - 1.850
R-factor	0.205
Rwork	0.204
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d3j
RMSD bond length	0.015
RMSD bond angle	2.224
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.850	1.850
Rmerge	0.100	0.700
Number of reflections	17655
<I/σ(I)>	16.3	1.4
Completeness [%]	98.8	91.3
Redundancy	5.3	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		PEG 8000 30 % W/V, 200 MM SODIUM ACETATE, 100 MM SODIUM CACODYLATE PH 6.5 .