2YGL

The X-ray crystal structure of tandem CBM51 modules of Sp3GH98, the family 98 glycoside hydrolase from Streptococcus pneumoniae SP3-BS71

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	76.040, 90.580, 119.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.910 - 2.100
R-factor	0.21142
Rwork	0.210
R-free	0.24671
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vnr
RMSD bond length	0.006
RMSD bond angle	0.931
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.100	0.380
Number of reflections	46386
<I/σ(I)>	22.9	7.3
Completeness [%]	99.7	99.7
Redundancy	13.6	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1