2YGH
SAM-I riboswitch with a G2nA mutation in the Kink turn in complex with S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 58.460, 58.460, 155.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.710 - 2.600 |
| R-factor | 0.2054 |
| Rwork | 0.200 |
| R-free | 0.25929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gx5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.892 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.800 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 8929 | |
| <I/σ(I)> | 22.6 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10 MM MGCL, 40 MM NA-CACODYLATE PH 7, 80 MM KCL, 16 MM SPERMINE-HCL, 14% (V/V) MPD. |
