2YG1
APO STRUCTURE OF CELLOBIOHYDROLASE 1 (CEL7A) FROM HETEROBASIDION ANNOSUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.511, 84.103, 75.877 |
| Unit cell angles | 90.00, 103.36, 90.00 |
Refinement procedure
| Resolution | 28.100 - 1.900 |
| R-factor | 0.20003 |
| Rwork | 0.197 |
| R-free | 0.25265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z3v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.230 |
| Number of reflections | 54342 | |
| <I/σ(I)> | 15.7 | 5.5 |
| Completeness [%] | 98.1 | 96.5 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.7 | PROTEIN WAS CRYSTALLIZED FROM 20 MM MGCL2, 0.1 M HEPES, PH 7.5, 22% POLYACRYLIC ACID 5100, NA SALT. |
