2YFP

STRUCTURE OF YELLOW-EMISSION VARIANT OF GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1997-12-05
Detector	SDMS
Spacegroup name	P 21 21 21
Unit cell lengths	51.994, 62.756, 69.929
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 2.600
Rwork	0.161
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.010
RMSD bond angle	20.510 *
Data reduction software	DATAC
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.780
High resolution limit [Å]	2.600	2.600
Rmerge	0.065	0.178
Total number of observations	29904 *
Number of reflections	7373
<I/σ(I)>	8.23	2.24
Completeness [%]	99.0	97
Redundancy	4.1	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	15 *	pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	50 (mM)
3	1	reservoir	sodium potassium phosphate	2.2 (M)