2YFO
GALACTOSIDASE DOMAIN OF ALPHA-GALACTOSIDASE-SUCROSE KINASE, AGASK, in complex with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 105.016, 111.603, 155.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.970 - 1.350 |
| R-factor | 0.12524 |
| Rwork | 0.124 |
| R-free | 0.14171 |
| Structure solution method | OTHER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.090 | 0.470 |
| Number of reflections | 198265 | |
| <I/σ(I)> | 11.8 | 3 |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 14% PEG 8000, 0.1M HEPES PH 7.5, PROTEIN CONCENTRATION 7MG/ML |
