2YDZ
X-ray structure of the cyan fluorescent protein SCFP3A (K206A mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.146, 62.140, 69.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.410 - 1.590 |
| R-factor | 0.1445 |
| Rwork | 0.142 |
| R-free | 0.19328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.410 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.060 | 0.830 |
| Number of reflections | 29189 | |
| <I/σ(I)> | 14.8 | 2 |
| Completeness [%] | 96.1 | 95.6 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.75 | 16 MG/ML PROTEIN, 13% PEG8000, 100 MM MGCL2, 100 MM HEPES PH 6.75 |
