2YDH
Crystal structure of the SAM-I riboswitch A94G U34 G18U G19U variant in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.899, 61.899, 157.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.620 - 2.900 |
| R-factor | 0.27406 |
| Rwork | 0.269 |
| R-free | 0.32145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gx5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.914 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 6875 | |
| <I/σ(I)> | 40.6 | 2.44 |
| Completeness [%] | 93.1 | 64.5 |
| Redundancy | 6.1 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 40 MM NA-CACODYLATE (PH 7.0), 12 MM SPERMINE-HCL, 12% (V/V) MPD, 80 MM KCL. |
