2YD5

Crystal structure of the N-terminal Ig1-2 module of Human Receptor Protein Tyrosine Phosphatase LAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 32 2 1
Unit cell lengths	76.950, 76.950, 68.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.475 - 2.200
R-factor	0.1973
Rwork	0.196
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yd4
RMSD bond length	0.004
RMSD bond angle	0.827
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.830
Number of reflections	12272
<I/σ(I)>	19.1	3.6
Completeness [%]	99.7	98.2
Redundancy	10.7	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		0.1 M SODIUM ACETATE TRIHYDRATE, 20% ISOPROPANOL, 20% (W/V) PEG 4000, 0.25 MM HEPARIN DP10, PH 5.6 .