2YD4
Crystal structure of the N-terminal Ig1-2 module of Chicken Receptor Protein Tyrosine Phosphatase Sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 104.745, 104.745, 94.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.070 - 1.650 |
| R-factor | 0.18079 |
| Rwork | 0.180 |
| R-free | 0.20229 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.421 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | SHELXD (VIA AUTOSHARP INTERFACE) |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.120 | 0.880 |
| Number of reflections | 31882 | |
| <I/σ(I)> | 26.2 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26 | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.6 | 20% W/V PEG 8000, 0.1M CHES, PH 9.6 . |
