2YD3
Crystal structure of the N-terminal Ig1-2 module of Human Receptor Protein Tyrosine Phosphatase Sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 104.218, 104.218, 94.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.957 - 2.300 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.887 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.140 | 0.660 |
| Number of reflections | 12003 | |
| <I/σ(I)> | 13.6 | 3.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 18.3 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 0.2M SODIUM SULPHATE, 20% W/V PEG 3350, PH 6.6 . |
