2YD2
Crystal structure of the N-terminal Ig1-2 module of Human Receptor Protein Tyrosine Phosphatase Sigma
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ASDC QUANTUM 4R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.400, 29.490, 61.580 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 27.237 - 2.552 |
| R-factor | 0.1995 |
| Rwork | 0.196 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yd4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.905 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.600 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 7438 | |
| <I/σ(I)> | 13.7 | 2.6 |
| Completeness [%] | 98.1 | 84.8 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 0.2M AMMONIUM IODIDE, 20% W/V PEG 3350, PH 6.2 . |
