2YCJ

methyltransferase bound with methyltetrahydrofolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43 21 2
Unit cell lengths	64.702, 64.702, 177.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.351 - 1.960
R-factor	0.2022
Rwork	0.200
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.329
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.010
High resolution limit [Å]	1.960	1.960
Rmerge	0.040	0.500
Number of reflections	27851
<I/σ(I)>	27.46	4.34
Completeness [%]	99.2	99.1
Redundancy	7.5	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1