2YCI

methyltransferase native

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43 21 2
Unit cell lengths	64.009, 64.009, 176.178
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.200 - 1.780
R-factor	0.19281
Rwork	0.191
R-free	0.23357
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.726
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.830
High resolution limit [Å]	1.780	1.780
Rmerge	0.030	0.560
Number of reflections	35890
<I/σ(I)>		2.79
Completeness [%]	99.4	99.7
Redundancy	7	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1