2YCD
Structure of a novel Glutathione Transferase from Agrobacterium tumefaciens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-13 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.200, 95.500, 88.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.766 - 1.400 |
| R-factor | 0.1732 |
| Rwork | 0.172 |
| R-free | 0.18650 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 41008 | |
| <I/σ(I)> | 19.9 | 4.1 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.3 | 1.4 M NA/K PHOSPHATE, PH 8.3 |
