2YBU
Crystal structure of human acidic chitinase in complex with bisdionin F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 144.781, 149.194, 151.278 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 2.250 |
| R-factor | 0.17343 |
| Rwork | 0.173 |
| R-free | 0.21916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lg2 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.100 | 0.580 |
| Number of reflections | 154470 | |
| <I/σ(I)> | 14.8 | 2.7 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 75% SATURATED NACL, 0.1 M HEPES PH 7.4 |
