2YB5

Structure of the fusidic acid resistance protein FusC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	110
Detector technology	CCD
Collection date	2010-08-04
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	34.150, 109.610, 61.150
Unit cell angles	90.00, 101.72, 90.00

Refinement procedure

Resolution	15.000 - 2.100
R-factor	0.21716
Rwork	0.213
R-free	0.28773
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.009
RMSD bond angle	1.108
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX (PHASER RESOLVE ARPWARP)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.700
Number of reflections	25179
<I/σ(I)>	15.4	2
Completeness [%]	98.0	97.6
Redundancy	7.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		20% ETHYLENE GLYCOL, 0.1M TRIS-HCL PH 8.0, 0.2M AMMONIUM ACETATE.
1		8		20% ETHYLENE GLYCOL, 0.1M TRIS-HCL PH 8.0, 0.2M AMMONIUM ACETATE.
1		8		20% ETHYLENE GLYCOL, 0.1M TRIS-HCL PH 8.0, 0.2M AMMONIUM ACETATE.