2YAS
HYDROXYNITRILE LYASE FROM HEVEA BRASILIENSIS COMPLEXED WITH RHODANIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.423, 106.771, 128.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 1.720 |
| R-factor | 0.1501 |
| R-free | 0.19140 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1yas |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.000 | 1.750 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.066 * | 0.237 * |
| Number of reflections | 34663 | |
| <I/σ(I)> | 18.9 | 5.8 |
| Completeness [%] | 98.7 | 99.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | PH=7.5 2 % PEG 400, 2.0 M AMMONIUM SULFATE, 20 DEGREES. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 2 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) |
