2YAP
X-ray induced reduction of laccase from Thermus thermophilus HB27 (62. 5-75.0 percent dose)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 93.537, 110.173, 96.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.869 - 1.800 |
| R-factor | 0.1506 |
| Rwork | 0.149 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xu9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.418 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.130 | 0.330 |
| Number of reflections | 44430 | |
| <I/σ(I)> | 8 | 4.1 |
| Completeness [%] | 96.7 | 97.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 70% MPD |
