2YAL
SinR, Master Regulator of biofilm formation in Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Collection date | 2009-10-09 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 36.117, 36.117, 250.342 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.720 - 2.270 |
| R-factor | 0.28921 |
| Rwork | 0.283 |
| R-free | 0.43475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BON (A 74-110 B) |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0108) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 4405 | |
| <I/σ(I)> | 59.6 | 2.6 |
| Completeness [%] | 85.7 | 42.4 |
| Redundancy | 12.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18% PEG MME2000, 0.16 M TRIS PH 8.5,0.01 M NI(II) CHLORIDE, 0.1 M MGCL2. |
