2Y9U
Structural basis of p63a SAM domain mutants involved in AEC syndrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.624, 38.309, 44.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.249 - 1.600 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.400 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.110 | 0.300 |
| Number of reflections | 8205 | |
| <I/σ(I)> | 14 | 4.7 |
| Completeness [%] | 98.9 | 96 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 100 MM SODIUM CITRATE, PH 6.4, 500MM LITHIUM SULPHATE, 500MM AMMONIUM SULPHATE, 5MM DTT |
