2Y9P
Pex4p-Pex22p mutant II structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.002, 41.630, 71.117 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 47.540 - 3.250 |
| R-factor | 0.20994 |
| Rwork | 0.207 |
| R-free | 0.26765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTY 2Y9M |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.221 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.540 | 3.420 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.100 | 0.200 |
| Number of reflections | 5245 | |
| <I/σ(I)> | 9.4 | 4.4 |
| Completeness [%] | 97.0 | 79.8 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 8.0 % (V/V) ETHYLENE GLYCOL, 14.0 % (W/V) POLYETHYLENE GLYCOL, 0.1 M HEPES PH 7.0 |
