2Y9N

Cellobiohydrolase I Cel7A from Trichoderma harzianum at 2.9 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	56.941, 69.259, 90.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.050 - 2.890
R-factor	0.22402
Rwork	0.222
R-free	0.26186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cel
RMSD bond length	0.003
RMSD bond angle	1.035
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.100
High resolution limit [Å]	2.900
Rmerge	0.120
Number of reflections	8493
<I/σ(I)>	6.5
Completeness [%]	99.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				35% PEG 4000.