2Y9F
High-resolution Structural Insights on the Sugar-recognition and Fusion Tag Properties of a Versatile b-Trefoil Lectin Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.580, 59.540, 61.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.160 - 1.470 |
| R-factor | 0.1593 |
| Rwork | 0.158 |
| R-free | 0.19372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w3a |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.315 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.550 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 22387 | |
| <I/σ(I)> | 16.9 | 5.4 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 6.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 12% (W/V) PEG 4000, 100 MM TRIS-HCL, PH 8.5, 150 MM SODIUM ACETATE |
