2Y93
Crystal Structure of cis-Biphenyl-2,3-dihydrodiol-2,3-dehydrogenase (BphB)from Pandoraea pnomenusa strain B-356.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-09 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.692, 75.692, 176.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.500 - 2.220 |
| R-factor | 0.18248 |
| Rwork | 0.181 |
| R-free | 0.21889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bdb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.500 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 26076 | |
| <I/σ(I)> | 13.6 | 2.4 |
| Completeness [%] | 97.0 | 40.6 |
| Redundancy | 5.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3-5 M SODIUM FORMATE |
