2Y90

Crystal structure of Hfq riboregulator from E. coli (P6 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 6
Unit cell lengths	61.500, 61.500, 28.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.261 - 2.252
R-factor	0.1881
Rwork	0.180
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk9
RMSD bond length	0.007
RMSD bond angle	1.164
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.000	2.300
High resolution limit [Å]	2.250	2.250
Rmerge	0.060	0.170
Number of reflections	2979
<I/σ(I)>	11.7	4.3
Completeness [%]	99.0	89.3
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL HANGING DROPS AT 20C. THE RESERVOIR CONTAINED 1.6 M AMMONIUM SULFATE, 0.1 M TRIS-HCL PH 8.0.