2Y8Y
Structure B of CRISPR endoribonuclease Cse3 bound to 19 nt RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.280, 71.210, 76.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.752 - 1.440 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y8w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.043 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.050 | 0.850 |
| Number of reflections | 44574 | |
| <I/σ(I)> | 22.56 | 2.29 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE, PH 5.0 4% PEG (W/V) 6000. |
