2Y8T
Co-structure of AMA1 with a surface exposed region of RON2 from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.860, 96.380, 78.350 |
| Unit cell angles | 90.00, 115.64, 90.00 |
Refinement procedure
| Resolution | 39.810 - 1.950 |
| R-factor | 0.173 |
| Rwork | 0.170 |
| R-free | 0.22804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2z |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.943 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.480 |
| Number of reflections | 68179 | |
| <I/σ(I)> | 10.6 | 3.7 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 1500, 100 MM MIB PH 4.0 . |
