2Y8R
Crystal structure of apo AMA1 mutant (Tyr230Ala) from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.520, 76.090, 88.780 |
| Unit cell angles | 71.89, 73.37, 73.50 |
Refinement procedure
| Resolution | 38.590 - 2.450 |
| R-factor | 0.21635 |
| Rwork | 0.212 |
| R-free | 0.30493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.622 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.590 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.100 | 0.460 |
| Number of reflections | 63415 | |
| <I/σ(I)> | 7.2 | 1.9 |
| Completeness [%] | 96.9 | 96.1 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 21% PEG3350, 100 MM TRIS PH 7.4 |
